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Table 1 Expression-invariant genes in E. coli during DNA damage as identified by the DP scheme and used for moose 2

From: RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study

Gene

Product

Process/Function

cysG a

uroporphyrin III C-methyltransferase

Other

dnaG

DNA primase

DNA replication

dtpB

dipeptide/tripeptide:H+ symporter DtpB

Transport

ftsX

Cell division protein ftsX

Cell division

ftsY

Cell division protein ftsY

Cell division

glyY

tRNAglyY

Translation

gyrB

DNA gyrase, subunit B

DNA replication

hcaT a

putative transport protein, major facilitator superfamily (MFS)

Transport

idnT a

L-idonate / 5-ketogluconate / gluconate transporter IdnT

Transport

ihfB a

integration host factor (IHF), beta subunit

Transcription

lhr

member of ATP-dependent helicase superfamily II

DNA replication

mutM

formamidopyrimidine DNA glycosylase

DNA repair

mutY

A/G-specific adenine glycosylase

DNA repair

ndk

nucleoside diphosphate kinase

Other

nfuA

iron-sulfur cluster scaffold protein

Other

pnp

polynucleotide phosphorylase monomer

RNA processing

rbbA

ribosome-associated ATPase

Translation

rhsB

RhsB protein in rhs element

Other

rpsU

30S ribosomal subunit protein S21

Translation

rrsA a

16S ribosomal RNA (rrsA)

Translation

rrsE

16S ribosomal RNA (rrsE)

Translation

rrsG

16S ribosomal RNA (rrsG)

Translation

secB

SecB chaperone

Protein localization

spoT

Guanosine-3′,5′-bis(diphosphate) 3′-pyrophosphohydrolase

Other

ssrA a

tmRNA

Trans-translation

tfaR

Rac prophage; predicted tail fiber assembly protein

Prophage

thrW

tRNAthrW

Translation

valS

Valyl-tRNA synthetase

Translation

yedJ

predicted phosphohydrolase

Other

ynaE

Rac prophage; cold shock protein, function unknown

Prophage

yphG

conserved protein

Unknown

zntA

zinc, cadmium and lead efflux system

Transport

zupT

heavy metal divalent cation transporter ZupT

Transport

  1. aEstablished reference genes in E. coli (see main text and Fig. 2b) were used as an input for moose 2