Updating microbial genomic sequences: improving accuracy & innovation

Table 2 Re-sequenced bacterial genomes from six organisms

Comparison of genomic sequencing quality between sanger & NGS methods
Organism	Genome size	First published year	Last updated year	Re-sequenced by illumina sequencer
Organism	Genome size	First published year	Last updated year	Coverage	Number of Inconsistent Loci
Brucelli suis 1330	Chr.1 2.1 M	2002	2011	1559X	12 loci
Brucelli suis 1330	Chr.2 1.2 M	2002	2011	1559X	12 loci
Caulobacter crescentus CB15	4.0 M	2001		325X	89 loci
Salmonella enterica ATCC 9150	4.6 M	2004		63X	17 loci
Bacteroides fragilis NCTC 9343	5.2 M	2002	2005	116X	6 loci
Bacillus halodurans C-125	4.2 M	2000	2005	111X	147 loci
Vibrio cholerae O1 N16961	Chr.1 2.9 M	2000		152X	165 loci
Vibrio cholerae O1 N16961	Chr.2 1.1 M	2000		152X	165 loci

The genome sequence of Brucella suis 1330 was re-sequenced using the Illumina GAIIx platform (previously published) and the additional five genomes were sequenced using Illumina MiSeq platform.

ISSN: 1756-0381