Luciferase reporter assay results. Reporter gene assay of pck2 400 bp fragment containing GCGGAGGC motif (A) and myogenin fragment containing CGACCCGT motif (B). There is a change in the reporter activity upon mutagenesis in pck2 constuct and there is no change in myogenin construct. Data normalized to corresponding myoblasts or myotubes transfected with pGL3 promoter vector in the case of pck2 assay (A), Myogenin data was normalized to myoblasts transfected with pGL4.15 containing myogenin construct, because pGL4.15 alone does not have any basal activity. Data represents at least three replicates ± s.e.m. (*, p < 0.05, t-test).