Table 2 Primers of ALDOA, CDC20, CDC45 and CCNB2 for qRT-PCR. Primer Premier 5.0 was used to design the primers, and Primer-BLAST tool of NCBI was used to ensure the accuracy and specificity of these primers
Targeted Gene | Primer | Sequence (5′–3′) |
|---|---|---|
ALDOA | Forward | ATGCCCTACCAATATCCAGC |
Reverse | GACAGCCCATCCAACCCT | |
CDC20 | Forward | GGCACCAGTGATCGACACATTCGCAT |
Reverse | GCCATAGCCTCAGGGTCTCATCTGCT | |
CCNB2 | Forward | GCGTTGGCATTATGGATCG |
Reverse | TCTTCCGGGAAACTGGCTG | |
CDC45 | Forward | TGGACTGCACACGGATCT |
Reverse | AACCTGGCTGCGGTATAG | |
TK1 | Forward | TGGCTGTCATAGGCATCGAC |
Reverse | CCAGTGCAGCCACAATTACG | |
β-Actin | Forward | AGCGAGCATCCCCCAAAGTT |
Reverse | GGGCACGAAGGCTCATCATT |