Fig. 5From: Elevated transcriptional levels of aldolase A (ALDOA) associates with cell cycle-related genes in patients with NSCLC and several solid tumorsEvaluation of ALDOA influence on cell cycle in SKBR3 under minimized glycolysis by flow cytometry. (a) WB indicated an obviously downregulated ALDOA expression in SKBR3-siALDOA compared to that in SKBR3-siNC. (b) Knockdown of ALDOA significantly increased the percentage of cells in G0/G1 phase (39.15 ± 4.75% vs. 50.32 ± 7.44%, P = 0.047), accompanied by a considerable decrease in the percentage of cells in S phase (35.41 ± 1.71% vs. 21.95 ± 2.80%, P = 0.006). No significant changes were seen in the percentage of cells in G2/M phase (19.40 ± 2.27% vs. 26.54 ± 5.15%, P = 0.112). (c) and (d), representative DNA histograms of SKBR3 transfected with siNC (SKBR3-siNC, C) or siALDOA (SKBR3-siALDOA, D) after incubation in glucose-free median for additional 8 hBack to article page