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Fig. 5 | BioData Mining

Fig. 5

From: Elevated transcriptional levels of aldolase A (ALDOA) associates with cell cycle-related genes in patients with NSCLC and several solid tumors

Fig. 5

Evaluation of ALDOA influence on cell cycle in SKBR3 under minimized glycolysis by flow cytometry. (a) WB indicated an obviously downregulated ALDOA expression in SKBR3-siALDOA compared to that in SKBR3-siNC. (b) Knockdown of ALDOA significantly increased the percentage of cells in G0/G1 phase (39.15 ± 4.75% vs. 50.32 ± 7.44%, P = 0.047), accompanied by a considerable decrease in the percentage of cells in S phase (35.41 ± 1.71% vs. 21.95 ± 2.80%, P = 0.006). No significant changes were seen in the percentage of cells in G2/M phase (19.40 ± 2.27% vs. 26.54 ± 5.15%, P = 0.112). (c) and (d), representative DNA histograms of SKBR3 transfected with siNC (SKBR3-siNC, C) or siALDOA (SKBR3-siALDOA, D) after incubation in glucose-free median for additional 8 h

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ISSN: 1756-0381