Upstream regulator | Predicted state | Activation score | Intersection P-value | Number of targets |
---|
IL4 | Activated | 2.115 | 2.115E-20 | 40 |
OSM | Activated | 2.616 | 2.41E-08 | 21 |
Stat5(A/B) | Activated | 2.630 | 6.50E-08 | 9 |
- IPA identified IL4 as the top upstream regulator of the \( {\boldsymbol{\gamma}}_1 \) signature that directly targeted 40 genes in the signature (Score=2.115, p=2.115E-20). These 40 genes formed a mRNA signature, φ
(40)IL4
, that was “anchored” upstream by IL4 with expression patterns that implied the up-regulation of this gene. Subsequent eigen-survival analysis shows that the φ
(40)IL4
signature was robustly associated with overall survival on the 291-sample discovery data set and a 99-sample independent test data set. Regulation of φ
(40)IL4
by IL4 linked overall survival of ovarian cancer patients with stage 3 disease to macrophage polarization in the tumor environment