Fig. 9

Cluster analysis by the K₁/k₂ signature reveals a novel subtype of HCC metabolically similar to ICC. a 2-way hierarchically clustered heatmap of K₁/k₂ signature in the 50-sample discovery data set. This analysis identified two distinct expression phenotypes Γ⁽⁻⁾ and Γ⁽⁺⁾ where Γ⁽⁻⁾ included samples where \( {\omega}_{mRNA}^{K_1/{k}_2} \) was down-regulated on the samples in Γ⁽⁻⁾ relative to the remaining samples in Γ⁽⁺⁾. The Γ⁽⁻⁾ class contained all 6 ICC samples plus 7 HCC and 2 sarcomas while Γ⁽⁺⁾ contained all 20 normal samples along with 15 HCC. b Plot of the dominant eigen-signal of the matrix for the \( {\omega}_{mRNA}^{K_1/{k}_2} \) signature clearly separates the samples in Γ⁽⁻⁾ and Γ⁽⁺⁾ based on a threshold set at zero