From: SePIA: RNA and small RNA sequence processing, integration, and analysis
Module | Component | Software | Reference |
---|---|---|---|
Preprocessing | Adaptor and quality trimming | FastX-Toolkit | |
 |  | Trimmomatic | [62] |
 |  | Trim Galore | |
 | Quality statistics | FastQC a | |
Read mapping | Align sequences to a reference | BWA | [63] |
 |  | Tophat | [64] |
 |  | Bowtie | [13] |
 |  | Bowtie2 | [65] |
 |  | STAR | [7] |
 | Alignment sorting and conversion | SAMtools a | [66] |
 |  | Picard tools a | |
 | Alignment statistics | RNA-SeQC | [67] |
 |  | RSeQC | [68] |
Expression | Mapped reads quantification | HTSeq | [69] |
 |  | Cufflinks | [70] |
Analysis | Variant calling | Bambino | [35] |
 | and annotation | ANNOVAR | [71] |
 | Differential expression | Cuffdiff | [18] |
 | R bioconductor packages for differential expression | DESeq | [15] |
 |  | DESeq2 | [72] |
 |  | DEXseq | [73] |
 |  | EdgeR | [14] |
 | novel miRNA discovery | miRanalyzer | [74] |
 |  | miRDeep2 | [75] |
miRNA-mRNA integration | R package for SQLite query | sqldf a | [76] |
 | Pathway impact analysis | SPIA | [77] |