Distinguishing highly similar gene isoforms with a clustering-based bioinformatics analysis of PacBio single-molecule long reads

Table 1 Reconstruction of an ~1.5 kb segment of 10 known msg isoforms of P. jirovecii from the benchmark isoform admixture by PacBio sequencing and clustering-based analysis (see Additional file 2: Table S2 and Additional file 3 for more details)

Contig no.	Length^a (bp)	Matched msg isoform	Identity (%)
Contig0001	1587	AR-Cl72	100
Contig0002	1581	AR-Cl59	99.94
Contig0003	1584	AR-Cl46	99.94
Contig0004	1584	AR-Cl14	99.87
Contig0005	1581	AR-Cl6	99.75
Contig0006	1581	AR-Cl24	99.87
Contig0007	1593	AR-Cl20	99.94
Contig0008	1584	AR-Cl44	99.81
Contig0011	1584	AR-Cl45	99.94
Contig0014	1583	AR-Cl58	99.87

ISSN: 1756-0381