Figure 7

Result Graph DS2. The reduced result graph for DS2 generated from TMHMM transmembrane-helical information. After removing less weighted edge connections, the graph is given more clearly. Different nodes are given as representative motifs. Two possible node colours describe the predicted topology state (TM = transmembrane, nTM = none-transmembrane) based on previous work by Grunert and colleagues [14] for each motif closer. This distinguishes TM-typical from TM-non-typical graph present motifs. Coloured weighted edges can be assigned to a occurrence of the pendant colour-scale. Ultimately, the graph makes clear how often different consecutive motifs occur. Highly occurring motifs are connected with red coloured edges. It is shown that always the same residues are recovered at the starting and ending position of a motif. Typical motifs with alanine, leucine, glycine or valine starting and ending residues are the representative structure forming motifs. Finally, often accrued motifs become apparent in their function as “hub”-motif. For example LL3, LV3, VL3 and AL3 often occur within a MA with other motifs. This leads to the indispensability to build helical regions within the membrane environment. The graph also shows motifs atypical for membrane environment. E.g. the topology prediction of SN3 and PY3 to ‘nTM’ state can be traced back to more ‘nTM’ conservation in evolution. This leads to the assumption, that these motifs are functionally important and relevant for family-specific functional characteristics of DS2.