Biological interpretation of color patterns identified in the animation-assisted network analysis. The left side shows the biological interpretation of the assayed conditions, where Hsp90 was inhibited (or not) in wild-type or p23 -deficient yeast strains. Genes showing significant fold change under these conditions are genes affected (directly or indirectly, positively or negatively) by Hsp90 and p23. On the right, the bar graphs illustrate the expected expression levels of any given gene in each condition of the pairwise comparisons, according to the observed final fold change (red or green colors). In the animation-assisted analysis (visualizing all four pairwise comparisons in a animation), there are genes which follow a defined behavior along all four comparisons, that is these genes display a defined pattern of red/green colors (according to their expression values). If a gene in the first two comparisons is first green and then red it means that the inhibition of Hsp90 leads to a decrease in its expression levels but the disruption of p23 results in an increase, which would be in accordance with a gene positively regulated by Hsp90 and negatively by p23. A similar reasoning can be applied to all other patterns.