From: Preprocessing differential methylation hybridization microarray data
Before MSRE digestion | After MSRE digestion | Probe signals |
---|---|---|
1). No MSRE cutting sites ATCGTCCAGCCGATTTAAACCCGTATCGTA | Not being restricted/cut, saved for hybridization | Contribute to the final probe signals |
2). All MSRE cutting sites are methylated ATCGC m G CCACCGATTTC m CGG TACGC m G GGAA | Not being restricted/cut, saved for hybridization | Contribute to the final probe signals |
3) At least one MSRE cutting site is not methylated ATCGCG CCACCGATTTC m CGG TACGCG GGAAA | Being cut and will not be hybridized onto the array | Do not contribute to the final probe signals |