MotifADE overview. Overview of MotifADE method: Genes are sorted by their moderated t-test statistic values. Motifs in the promoter regions in these genes are identified. Two-tailed Mann-Whitney rank sum statistics is applied. In this schematic view, Motif 1 is significant in the up-regulated genes. Motif 2 is not significant in either the up-regulated genes or down-regulated genes and Motif 3 is significant in the down-regulated genes.