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Figure 2 | BioData Mining

Figure 2

From: Multivariate profiling of neurodegeneration-associated changes in a subcellular compartment of neurons via image processing

Figure 2

Preparation of Drosophila larvae for imaging labelled endolysosomal compartments in nerve termini. Third instar larvae are dissected and opened out as indicated (upper left) to reveal the brain and larval motorneurons with axons (green) extending into the peripheral muscle field, with segments labelled T2-A7 (purple; lower left). A bundle of axons, including the aCC and RP2 motoraxons (see text) and expressing membrane-localized EGFP is shown making branching contacts with dorsal muscle fibers (lower right) in an actual fixed and immunostained preparation of a larval body wall. The nerve terminal (or neuromuscular junction, NMJ) is boxed and the equivalent area of an animal expressing spinster-GFP is shown (upper right), highlighting the punctate endolysosomal compartments in the aCC terminal.

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ISSN: 1756-0381